How is IVF done step by step?

Step-by-step through an IVF cycle

An IVF ‘cycle’ is how we describe one round of IVF treatment starting with the first day of your period. Check out IVF Treatment Process in India step by step with more details.

As part of your fertility plan, you may start medication or injections before the first day of the cycle.

IVF cycle step by step by Doctors Review India

Step 1: Day 1 of your period

The first official day of your IVF treatment cycle is day 1 of your period. Everyone’s body is different, and your fertility nurse will help you understand how to identify day 1.

Step 2: Stimulating your ovaries

The stimulation phase starts from day 1. In a natural monthly cycle, your ovaries normally produce 1 egg. You’ll take medication for 8-14 days to encourage the follicles in your ovaries (where the eggs live) to produce more eggs.

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Your specialist prescribes medication specific to your body and treatment plan. It’s usually in the form of injections, which can vary from 1-2 for the cycle, or 1-2 per day. It can be daunting, but your fertility nurse will be there to show you exactly how and where to give the injections. You can get your partner involved too and watch and learn together to get it right. It quickly becomes a habit and you’ll be an expert in no time.

The most common hormones in the medications used to stimulate the follicles are:

• follicle-stimulating hormone (FSH)
• luteinizing hormone (LH).

We keep an eye on your ovaries and how the follicles are developing with blood tests and ultrasounds. Your medication will be adjusted if needed. You will have some transvaginal ultrasounds (a probe is inserted internally). Our team will support you through these processes and make you as comfortable as we can.

We’ll track you more frequently towards the end of the stimulation phase to time the ‘trigger injection’ perfectly.

The trigger injection gets the eggs ready for ovulation – the natural process where eggs are released and you have your period. Your fertility nurse tells you exactly when to do the trigger injection. Your fertility specialist will schedule the egg retrieval before you ovulate.

Step 3: Egg retrieval

Egg retrieval, or egg ‘pick up’, is a hospital day procedure where the eggs are collected from your ovaries. An anaesthetist will get you ready for a general anaesthetic. You’ll be asleep and the procedure takes about 20-30 minutes.

Your fertility specialist uses the latest ultrasound technology to guide a needle into each ovary. It’s delicate work where every millimetre counts, and this is where the experience of our specialists pays off. You can’t see an egg with the naked eye; they’re contained in the fluid within the follicles in your ovaries. The specialist removes fluid from the follicles that look like they’ve grown enough to have an egg inside.

Your fertility specialist should have a fair idea from your ultrasounds how many eggs there are before retrieval. The average number of eggs collected is 8-15.

Recovery takes about 30 minutes and you’ll be able to walk out on your own. It’s a good idea to have a support person with you as you won’t be able to drive after the procedure.

Step 4: The sperm

If you’re a couple planning on using fresh sperm, the male will produce a sample the morning of the egg retrieval. If you are using frozen or donor sperm, our scientists will have it ready in the lab.

The sperm is graded using 4 different levels of quality. It’s washed in a special mixture to slow it down so our scientists can spot the best ones under the microscope. A perfect, healthy sperm is not too fat or thin, with a tail that’s not too long or short. The best sperm are selected, and they’re ready and waiting in the lab to be introduced to the eggs.

Step 5: Fertilisation

Your fertility specialist gives our scientists the eggs they have retrieved, still in the fluid from the follicles of the ovaries. The scientists use powerful microscopes to find the eggs in the fluid so they can be removed.

It’s important the eggs are fertilised quickly. The eggs and some sperm are placed in a dish. They have the chance to find each other and fertilise like they would naturally within your body.

Step 6: Embryo development

If the sperm fertilises the egg, it becomes an embryo. Our scientists put the embryo into a special incubator where the conditions for growth and development are perfect.

We create the perfect growing conditions using a mix of amino acids, just like your body would use to nurture the embryo.

Our scientists keep an eye on the embryos over 5-6 days. What we want is:

a two- to four-cell embryo on day 2 and a six- to eight-cell embryo on day 3 (called the cleavage stage)
We know implanting embryos at the blastocyst stage into the uterus boosts your chances of a successful pregnancy.

Unfortunately, not all eggs will fertilise and reach embryo stage. The eggs might not be mature or the sperm not be strong enough. We know you’ll be waiting on news, so we’ll keep you up to date with the progress of your egg, sperm and embryo development.

Stage 7: Embryo transfer

If your embryo develops in the lab, you’re ready for it to be transferred into your uterus.

Your fertility nurses will contact you to explain what you will need to do to prepare.

The embryo transfer is a very simple process, like a pap smear. It takes about 5 minutes, you’ll be awake, there’s no anaesthetic, and you can get up straight away. You can continue with your day, the embryo can’t fall out if you stand up or go to the toilet.

A scientist prepares your embryo by placing it in a small tube called a catheter. It’s critical this is done by an expert to disturb the embryo as little as possible.

Your fertility specialist places the catheter through your cervix and into your uterus.

Step 8: The final blood test

Approximately two weeks after your embryo transfer, you’ll have a blood test to measure your levels of the hormone hCG (human chorionic gonadotropin). hCG in your bloodstream usually means a positive pregnancy test. Your nurse will let you know exactly when you need to have your blood test, as it may vary for some patients.

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March 11, 2020